Product & Services > Conjugation Service
Flogen Conjugation Service
Details
Custom Conjugation | Ordering Instructions
We offer high quality, efficient and cost-saving conjugation services
for your precious protein with fluorescent dyes, phycobiliproteins,
biotin, enzymes or other molecules. Our self-produced phycobiliproteins (R-PE, B-PE, APC, cl-APC, and CPC) have been sold to world-renowned companies for years. Its quality is well controlled and the property is also well studied for conjugation.
Every protein has its own unique characteristics, so it is essential to conjugate accordingly to reach optimization. Our conjugation services include screening and selecting the best phycobiliproteins, and using minimum amount of protein to find the best yield and the most optimal conjugation form for our customers. Our conjugation service can minimize your costs and provide a high quality conjugate product with 100% satisfaction!
Basically, we complete the conjugation order within a week from the time we receive the protein from you. In order to optimize the conjugation conditions, 0.2mg to 1mg of the chosen protein will be tested and HPLC analyzed prior to mass labeling.? The sample tested under optimization conditions will be mixed with the mass conjugate product for final purification.
Small Amount Trial Schedule: For 3 clones
Step |
Company X |
FEBICO |
Shipping & Confirmation |
Ship 30 mg bulk and 2 other trial samples (at least 1 mg each) |
Confirm receipt of your proteins by email. Shipment check. |
Preparation |
Charge NT$500/clone if extra preparation is needed |
Pretreatment to conjugation ready conditions (Ab. conc. > 2mg/ml in PBS without NaN3). |
Quantification |
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Protein amount analysis by spectrometry. |
Conjugate
condition testing |
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Optimization Conditions Study with 2 different methods (use 0.25~1mg Ab for each clone).
Use HPLC analysis to estimate possible bulk conjugation yield. |
Shipping &
Confirmation |
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Provide 0.1mg Ab-RpE
(2 methods/clone) for evaluation.
Clone1 A&B and Clone2 A&B and Clone3 A&B, 6 samples in total. |
Evaluation |
1. After receiving samples for bulk Ab,? Company X needs to choose either Method A or Method B (preferably within 3 days).
2. As soon as Company X selects a production method, FEBICO will start the production schedule.
3. Provide samples evaluation data for 3 clones. |
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Bulk Production Schedule: for 30 mg Ab and under
Step |
Company X |
FEBICO |
Conjugation |
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Process bulk conjugation by your chosen method. |
Purification |
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Separate un-conjugated proteins
from conjugated proteins by chromatographic gel. |
Fraction and final
product analysis |
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Measure fraction and final product by spectrophotometer. |
Packing and COA
preparation |
Wire payments by designated date |
E-mail COA and invoice (Charge based on final recovery of Ab conjugate) to Company X. |
Shipping |
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Ship products after confirmation of payment. |
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Custom Conjugation <back to top
Requirement of your protein:
- Free of carrier proteins, amine-containing buffers, salt, and ammonium?sulfate.
* Removal of those from your protein will be charged with additional cost.
- Quantity: at least 1mg (2mg for phycobiliprotein conjugation)
- Concentration: at least 2mg/ml
Conjugation Pricing <back to top
For exact pricing and customization, please fill out the Custom Protein Conjugation Form Sheet, and send it to service@febico.com.tw or fax it to 886-2-2655-8196.
Be sure to leave contact information including email. We will contact you as soon as possible.
Ordering Instructions: <back to top
Please Fax or email your order to FEBICO:
Fax number: +886-2-2655-8196
Email: service@febico.com.tw
Ship your proteins to:
Room A, 13FL, No.3, Yuan-Chiu St., Nan-Gang District,
Taipei, Taiwan, R.O.C.
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