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MSEP-EX Cell Culture Supplement, Lyophilized
NumberingMSEP-EX
Product Overview
MSEP-EX is an animal-derived component-free (ADCF) cell culture supplement designed to reduce the use of fetal bovine serum (FBS) and human platelet lysate (hPL). When added to the culture medium, it can replace up to 80% of serum without requiring cell adaptation. MSEP-EX supports the growth of various mammalian cell types under low-serum conditions across multiple passages, including myoblasts, fibroblasts, epithelial cells, T cells, and mesenchymal stem cells. Additionally, it can be used as a booster in chemically defined media to enhance protein production during cell culture. Extracted from edible plants, MSEP-EX is ethical-friendly and carries a lower risk of pathogen contamination compared to animal-derived products. Offering cost-efficiency, high performance, broad versatility across cell types, and adaptation-free use, MSEP-EX is an ideal supplement for biologic production.
Applications
Applications
- Biologics Production
- Cell Culture Medium Supplement
- Animal Serum Reduction
Instruction to Use
Reconstituting MSEP-EX with basal media intended to use. Incubate at 37 °C for 30 minutes to dissolve MSEP-EX completely. Gently pipetting to homogenize MSEP-EX solution before use. It’s normal if precipitation appeared in MSEP-EX solution after long term storage.
Packing size
20mg/vial
Reconstituting MSEP-EX with basal media intended to use. Incubate at 37 °C for 30 minutes to dissolve MSEP-EX completely. Gently pipetting to homogenize MSEP-EX solution before use. It’s normal if precipitation appeared in MSEP-EX solution after long term storage.
Packing size
20mg/vial
Figures
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Figure 1. Comparable cell growth profiles between 10% FBS and 2% FBS + MSEP-EX supplement in DMEM are measured after 72 hours incubation
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Figure 2. Higher yield of protein production in chemical defined medium with MSEP-EX supplement are measured in CHO cell culture.
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Figure 3. Comparable cell double time in 1% MSEP-EX supplement group in both BM-MSC and UC-MSC cell culture.
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Figure 4. MSEP-EX supplement increases aging T cell proliferation in primary T cell culture
Features
Source
Plant-based extract
Form
Lyophilized powder
Color
Light yellow to pale white
Solubility (Turbidity)
About 6 mg/mL in sterile water. Slight cloudy in water solution.
Reconstitution
Reconstitute in sterile basal media intended to use with indicated volume.
Recommend Final Concentration: 4 mg/mL
Working Concentration
4 to 40 ug/mL of MSEP-EX as supplement in culture medium is recommended
Shipping
Shipped at room temperature.
Stability and Storage
2 years, below 35 °C as lyophilized powder
2 weeks, 2°C to 8 °C under sterile conditions after reconstitution
Plant-based extract
Form
Lyophilized powder
Color
Light yellow to pale white
Solubility (Turbidity)
About 6 mg/mL in sterile water. Slight cloudy in water solution.
Reconstitution
Reconstitute in sterile basal media intended to use with indicated volume.
Recommend Final Concentration: 4 mg/mL
Working Concentration
4 to 40 ug/mL of MSEP-EX as supplement in culture medium is recommended
Shipping
Shipped at room temperature.
Stability and Storage
2 years, below 35 °C as lyophilized powder
2 weeks, 2°C to 8 °C under sterile conditions after reconstitution