MSEP-EX Cell Culture Supplement, Lyophilized
Product Overview
MSEP-EX is an animal-derived component-free (ADCF) cell culture supplement designed to reduce reliance on fetal bovine serum (FBS) and human platelet lysate (hPL). When added to culture medium, MSEP-EX can replace up to 80% of serum without requiring cell adaptation, supporting consistent performance across multiple passages.
Extracted from edible plants, MSEP-EX carries a lower risk of pathogen contamination compared to animal-derived alternatives, making it an ethical and cost-effective choice for biologic production.
Validated cell types include: CHO, RD, MRC5, myoblasts, fibroblasts, epithelial cells, T cells, and mesenchymal stem cells (BM-MSC, UC-MSC).
Applications
- Biologics Production
- Virus Production (e.g. enterovirus, lentivirus)
- Protein Expression Enhancement
- Cell Culture Medium Supplement
- Animal Serum Reduction (FBS / hPL)
Key capabilities
- Reduces FBS or growth factor usage by 50% or more without cell adaptation
- Promotes cell proliferation across diverse mammalian cell types
- Enhances protein yield in chemically defined media
- Increases virus production titers (enterovirus, lentivirus)
- Supports stem cell culture with maintained doubling time across passages
Instruction to Use
Reconstitute with the basal medium of choice. Recommended working concentration: 4–40 µg/mL. Please refer to the Technical Data Sheet for full reconstitution and usage instructions.
Packing size
20 mg/vial. Other sizes available upon request. Please contact us.
Figures
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Figure 1. Comparable cell growth profiles between 10% FBS and 2% FBS + MSEP-EX supplement in DMEM are measured after 72 hours incubation
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Figure 2. Higher yield of protein production in chemical defined medium with MSEP-EX supplement are measured in CHO cell culture.
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Figure 3. Comparable cell double time in 1% MSEP-EX supplement group in both BM-MSC and UC-MSC cell culture.
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Figure 4. MSEP-EX supplement increases aging T cell proliferation in primary T cell culture
Features
Form — Lyophilized powder
Color — Light yellow to pale white
Solubility (Turbidity) — About 6 mg/mL in sterile water. Slightly cloudy in aqueous solution.
Reconstitution — Reconstitute in the sterile basal medium of choice. Recommended stock concentration: 4 mg/mL
Working Concentration — 4 to 40 µg/mL as supplement in culture medium
Packing Size — 20 mg/vial. Custom packing sizes available upon request — please contact us.
Shipping — Shipped at room temperature.
Stability and Storage — 2 years, below 35°C as lyophilized powder / 2 weeks, 2°C to 8°C under sterile conditions after reconstitution.
