Streptavidin-FITC Conjugate (SA-FITC)

Streptavidin-FITC (SA-FITC) is a high-quality fluorescent conjugate produced from our own in-house streptavidin under strict quality control. Supplied in 0.1 M sodium phosphate buffer (pH 7.2) with 0.09% sodium azide, it offers excellent stability and consistent performance across lots.

FITC is covalently conjugated to streptavidin at an optimized molar ratio — strong enough for bright, reliable detection, yet carefully controlled to preserve all four biotin-binding sites on the native tetrameric streptavidin structure.

Features and Advantages

• Consistent Lot-to-Lot Quality — Tight CV across production batches ensures reproducible results in your assays
• In-House Raw Materials — Streptavidin is produced and purified in-house, giving us full control over conjugation input quality
• Strict QC Criteria — Every lot tested for biotin-binding activity and fluorescence intensity before release
• Optimized FITC:Protein Labeling Ratio — Maximizes fluorescence signal while preserving streptavidin's four-site biotin-binding activity
• Flexible Production Capacity — Large-scale manufacturing capability to support OEM, diagnostic kit, and research supply needs
• Customizable Specifications — Custom FITC:protein ratios, buffer formulations, and packaging sizes available upon request

Applications

SA-FITC is excitable at 488 nm (standard laser line) and emits at a fluorescence maximum of 521 nm, placing it squarely in the FITC/FL1 detection channel. It is widely used in:

• Flow Cytometry — Detection of biotinylated antibodies and other biotinylated molecules in immunophenotyping and cell sorting workflows
• Fluorescence Immunoassay (FIA) — Rapid, sensitive signal generation for diagnostic and research platforms
• Immunohistochemistry (IHC) / Immunofluorescence (IF) — Amplified tissue and cell staining using biotin-streptavidin systems
• ELISA and Lateral Flow — Sandwich assay signal amplification
• Microarray and Bead-Based Assays — Multiplexed detection on Luminex and similar platforms