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Streptavidin RPE
Numbering
Product Overviews
Biotinylated nucleic acid or biotinylated antibody can be detected by streptavidin-fluoroprobe conjugate. Streptavidin (SA) is a protein that is capable of binding four biotins with a very high affinity. Phycoerytherin (PE) is extracted from red algae and express strong fluorescent intensity with absorption max at 496, 546, and 565 nm with max emission at 578 nm.
Streptavidin-RPE (SAPE) is commonly used in biotin-streptavidin detection protocols developed for flow cytometry, beads (ex, Luminex xMAP®, illumine Veracode®), microarray (ex. Affymetrix GeneChip®), IHC (Immunohistochemistry) and microplate assays.
Features and Advantages
Consistent Lot-to-Lot Quality
Self Made Fresh and High Quality Materials for Conjugation
Strict QC Criteria
Biotin-Binding and Fluorescent Intensity Check
Flexible Production Capacity
Well-Experienced Team for Conjugation
Unique Process for Fast and Large-Scale Purification
Application
For Flow Cytometry
Streptavidin-based amplification techniques are widely used in flow cytometry for increased signal output and greater sensitivity. Phycobiliprotein-conjugated streptavidin such as SA-PE, and SA-clAPC are used to detect biotinylated biomolecules such as primary and secondary antibodies.
For Microarray
Array of oligonucleotides is imprinted to the gene chips. Gene expression can be detected via labelling biotin to cRNA and allow hybridization occurs with the target. Staining with SAPE to allow expression of PE fluorescent intensity and analyzed by software.
For beads or microsphere (ex. Luminex xMAP®, illumina® Vera Code)
Beads coupling with carboxyl group or amine linker can be coupled to antibody or nucleic acid. Target protein or target complimentary oligonucleotide is biotinylated and allow agglutination or hybridization occurred with antibody or nucleic acids bound to the beads. Using a reporter molecule (ex. SAPE) bind with the biotinylated target, target can be detected by the fluorescent
intensity of PE.
Application
For Flow Cytometry
Streptavidin-based amplification techniques are widely used in flow cytometry for increased signal output and greater sensitivity. Phycobiliprotein-conjugated streptavidin such as SA-PE, and SA-clAPC are used to detect biotinylated biomolecules such as primary and secondary antibodies.
| Label | Excitation | Emission |
| R-Phycoerytherin (RPE) | 496, 546, 565 | 578 |
| Crosslinked APC (cl-APC) | 650 | 660 |
| FITC | 490 | 525 |
For Microarray
Array of oligonucleotides is imprinted to the gene chips. Gene expression can be detected via labelling biotin to cRNA and allow hybridization occurs with the target. Staining with SAPE to allow expression of PE fluorescent intensity and analyzed by software.
For beads or microsphere (ex. Luminex xMAP®, illumina® Vera Code)
Beads coupling with carboxyl group or amine linker can be coupled to antibody or nucleic acid. Target protein or target complimentary oligonucleotide is biotinylated and allow agglutination or hybridization occurred with antibody or nucleic acids bound to the beads. Using a reporter molecule (ex. SAPE) bind with the biotinylated target, target can be detected by the fluorescent
intensity of PE.
Figures
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Results indicated Flogen SAPE has an nearly idential mean fluorescent intensity in the sandwich immunoassay as Invitrogen SAPE. -
Flogen SAPE was tested against Invitrogen using DNA direct hybridization as model assay system. Result indicated Flogen SAPE is highly correlated to the expression of Invitrogen SAPE, with R2 value = 0.9853.
References and Citations
- Protein Biomarkers of Cardiovascular Disease and Mortality in the Community. (n.d.). ResearchGate. Retrieved August 18, 2022, from https://www.researchgate.net/publication/326375426_Protein_Biomarkers_of_Cardiovascular_Disease_and_Mortality_in_the_Community/fulltext/5b48a3faa6fdccadaec770c6/Protein-Biomarkers-of-Cardiovascular-Disease-and-Mortality-in-the-Community.pdf
TDS/COA/MSDS
| Cat. No. | Product | TDS | COA | MSDS |
|---|---|---|---|---|
| 1SA (I, II, III, IV, V) | Streptavidin-RPE Conjugate (Type I, II, III, IV, V) |
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